Spring 2009

ECE598:  SPECIAL TOPICS IN LIGHT MICROSCOPY

Prof. Gabriel Popescu

 

Text (optional): “Fundamentals of Light Microscopy and Electronic Imaging”, D. B. Murphy, 2001 (Wiley-Liss, 1st edition)

Schedule: 14-15:20 T-R, 241 Everitt Lab

Office Hours: 2-4 Fridays, 4055 Beckman;

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REGISTER NOW for the 2009 Nano-Biophotonics Summer School

 

Grading formula: Midterm 20%, Homework 20%, Project Presentation 20%, Project Report 40%, Class participation 10% (bonus)

Date

Comments

Topic

Video recordings

Supporting Material

Homework

Links

Jan 20

Introduction

 

 

Math review (ECE460)

Jan 22

Math review

Lecture 2

Appendix: Math review

 

Review 1: Microscopy of cells;  Review 2: Nanoscopy

Jan 27

No lecture

 

Microscopy from the beginning (Zeiss)

Jan 29

No lecture

 

 

Fourier pairs- Wikipedia

Feb 03

Properties of Light

Lecture 3

1. Properties of light

Optics puzzle

Feb 05

Properties of Light

Lecture 4

Electromagnetic spectrum- Wikipedia

Feb 10

Statistical Optics

Lecture 5

2. Statistical optics

 

R. J. Glauber- Nobel Lecture

Feb 12

Gaussian Optics

Lecture 6

3. Gaussian optics

Fermat’s Principle- Wikipedia

Feb 17

Gaussian Optics

Lecture 7

HW 1;  Sol. 1

Carl Friedrich Gauss- Wikipedia

Feb 19

Fourier Optics

Lecture 8

4. Fourier Optics

Joseph Fourier- Wikipedia; Fourier transform- Wolfram

Feb 24

Fourier Optics

Lecture 9

 

2D Fourier transforms

Feb 26

Elastic light scattering

 

Lecture 10

5. Elastic light scattering

ImageJ- free download; Image processing fundamentals

Mar 03

Elastic light scattering

Lecture 11

Scattering- Wikipedia

Mar 05

Dynamic Light Scattering

Lecture 12

6. Dynamic Light Scattering

HW 2;  Sol. 2

DLS- Wikipedia

Mar 10

Dynamic Light Scattering

Lecture 13

Einstein’s paper on Brownian motion

Mar 12

Microrheology

Lecture 14

 

Mar 17

Exam review

 

HW 3;  data

Mar 19

Midterm

 

Mar 24

Break

 

 

Mar 26

Break

 

 

Mar 31

Traditional Microscopy (intrinsic contrast)

 

7. Microscopy

 

Microscopy- Wikipedia

Apr 02

Traditional Microscopy (extrinsic contrast)

 

 

Apr 07

Interference microscopy

 

8. Review of Nonlinear Optics

 

Nikon- MicroscopyU

Apr 09

Quantitative Phase Microscopy

 

 

Apr 14

1.       CARS by Charles Fang

·         ISAM by Dr. Steven G. Adie (cancelled)

·         MagnetoMotive Optical Coherence Tomography by Dr. Renu John

 

Peer evaluation form- new

 

Apr 16

2.       Digital holographic microscopy by Santoshi Tripathi

3.       FRET by Chun Ge

4.       STORM by Sungwan Shin

 

 

Apr 21

5.       Deconvolution microscopy by Zhuo Wang

6.       SHGM by Daren Whitlock

 

 

Apr 23

7.       NSOM by Kashif Altaf

8.       Nonlinear structured illumination microscopy by Raghu Ambekar

9.       TIRF by Mustafa Mir

 

 

Apr 28

10.    STED by Budiman Dabarsyah

11.    FIONA by Markita Landry

·         Evaluation forms

 

 

Apr 30

12.    Optical coherence microscopy (OCM) by Shu Zhang

13.    Fluorescence correlation spectroscopy (FCS) by Ru Wang

14.    Single-molecule high-resolution colocalization (SHREC) by Vikram Chaudhery,

 

 

May 05

15.    Apertureless NSOM (ANSOM) by Wladimir Benalcazar

·         Fabrication of Photonic Crystals Using Holographic Lithography Method by Dr. Jun Xu

·         ISAM by Dr. Steven G. Adie

 

 

May 07

Reading Day

 

 

May 12

Reports Due

 

 

May 14